Don’t Eat Dirt Though!

A teaspoon of dirt contains an estimated 10,000 species of bacteria, but it’s only one percent of these microbial bugs — the ones that can be grown easily in a lab — that have brought us antibiotics, anticancer agents and other useful drugs.

The odds favor the other 99 percent for clinical promise, too, but scientists have had little success in tapping this unknown majority for new medicines because of the difficulty of analyzing the bugs’ DNA.

Now researchers at The Rockefeller University have extracted that genetic material from a lump of earth and turned it into an environmental DNA “megalibrary” that may provide access to many previously unknown organic compounds. The library has already led them to the genetic code for two potential antibiotics; the scientists also used enzymes from one set of cloned genes to produce new antibiotic derivatives as powerful as the strongest drugs we have today.

The research could recharge interest in the search for new compounds in the environment that has flagged over the past decade because of lackluster results. The new findings suggest that all sorts of useful and unknown products are being manufactured by bacteria in the soil that we routinely trample underfoot. And it shows a promising way to get at them.

“This proves that you can recover large gene clusters from very complex soil samples,” says Sean F. Brady, who heads the Laboratory of Genetically Encoded Small Molecules. “It suggests that there’s an untapped reservoir out there of variations on many clinically relevant antibiotics, and that by using culture-independent screening strategies, we may be able to gain access to them.”

The search for new antibiotics is increasingly urgent as “drugs of last resort” like vancomycin and teicoplanin are resorted to more commonly these days. Some of the meaner strains of Staphylococcus and other infections, including tuberculosis, have developed resistance to traditional antibiotics after decades of exposure to them.

By heating up about a cupful of soil in the presence of a detergent, Brady and graduate student Jacob Banik were able to separate the DNA from the dirt and scan the genetic material for a gene sequence called OxyC that is common to the family of antibiotics including vancomycin and teicoplanin. In soil samples from Pennsylvania, New Jersey, Massachusetts, Utah, Oregon, North Carolina, Tanzania and Costa Rica, they found that each contained at least one previously undiscovered variant of the OxyC sequence.

more via sciencedaily