Chemistry professor Yi Lu led a team that developed a technique that allows DNAzymes to cut double-stranded DNA, enabling a wide range of genetic engineering applications.
by Liz Ahlberg Touchstone , University of Illinois at Urbana-Champaign
Move over, gene-editing proteins—there’s a smaller, cheaper, more specific genetic engineering tool on the block: DNAzymes—small DNA molecules that can function like protein enzymes.
Researchers at the University of Illinois Urbana-Champaign have developed a technique that, for the first time, allows DNAzymes to target and cut double-stranded DNA, overcoming a significant limitation of the technology. DNAzymes have been used in biosensing, DNA computing and many other applications. However, when it comes to genetic engineering applications such as gene editing or gene therapy, they have faced a challenge: DNAzymes have only been able to target sites on single-stranded DNA, while the DNA coding for genes in cells is double-stranded. The researchers published their new technique in the Journal of the American Chemical Society.
Continue reading… “DNAzymes could outperform protein enzymes for genetic engineering”