In this sequence, a spinach leaf is stripped of its plant cells, a process called decellularization, using a detergent. The process leaves behind the leaf’s vasculature. Researchers at Worcester Polytechnic Institute (WPI) were able to culture beating human heart cells on such decelluralized leaves. Credit: Worcester Polytechnic Institute
Researchers face a fundamental challenge as they seek to scale up human tissue regeneration from small lab samples to full-size tissues, bones, even whole organs to implant in people to treat disease or traumatic injuries: how to establish a vascular system that delivers blood deep into the developing tissue.
Current bioengineering techniques, including 3-D printing, can’t fabricate the branching network of blood vessels down to the capillary scale that are required to deliver the oxygen, nutrients and essential molecules required for proper tissue growth. To solve this problem, a multidisciplinary research team at Worcester Polytechnic Institute (WPI), the University of Wisconsin-Madison, and Arkansas State University-Jonesboro have successfully turned to plants. They report their initial findings in the paper “Crossing kingdoms: Using decelluralized plants as perfusable tissue engineering scaffolds” published online in advance of the May 2017 issue of the journal Biomaterials.