Aligned myotubes formed on electrospun extracellular matrix scaffolds produced at Rice University. The staining with fluorescent tags shows cells’ expression of myogenic marker desmin (green), actin (red) and nuclei (blue) after seven days of growth. Credit: Mikos
Original story from Rice University
Rice University bioengineers are fabricating and testing tunable electrospun scaffolds completely derived from decellularized skeletal muscle to promote the regeneration of injured skeletal muscle.
Their paper in Science Advances shows how natural extracellular matrix can be made to mimic native skeletal muscle and direct the alignment, growth and differentiation of myotubes, one of the building blocks of skeletal muscle. The bioactive scaffolds are made in the lab via electrospinning, a high-throughput process that can produce single micron-scale fibers.
The research could ease the burden of performing an estimated 4.5 million reconstructive surgeries per year to repair injuries suffered by civilians and military personnel.
Current methods of electrospinning decellularized muscle require a copolymer to aid in scaffold fabrication. The Rice process does not.
“The major innovation is the ability to prepare scaffolds that are 100% extracellular matrix,” said Rice bioengineer and principal investigator Antonios Mikos. “That’s very important because the matrix includes all the signaling motifs that are important for the formation of the particular tissue.”
Continue reading… “Bio-Inspired Scaffolds Help Promote Muscle Growth”